05.02.2010 - Publication in TECAN Journal
Automated DNA preparation pushes forward evolutionary biology research
Reliable high throughput genotyping has helped research in parenthood analysis and population genetics of the three-spined stickleback at the Max Planck Institute of Evolutionary Biology, Plön, Germany.
Read the article in TECAN Journal and download the application note:
Article (96KB)
Complete Journal (2.9 MB)
Invitek Application Note (699KB)
The corresponding application note describes the high-throughput isolation of genomic DNA from different populations of three-spined stickleback as well as from three-spined stickleback eggs using the Invisorb DNA Tissue HTS 96 Kit (link to product page) and the vacuum based robotic workstation Freedom Evo 200 from Tecan. In this experiment up to 96 samples were processed on the robotic platform in an automated operation. The isolated DNA was used for microsatellite analysis for parenthood analysis and population genetics. The results show that the extracted genomic DNA can be used for high scale genotyping and genotyping of adaptive and ecologically relevant genes such MHC genes in evolutionary biology.
