Invisorb® DNA Tissue HTS 96 Kit/ Tec

The Invisorb DNA Tissue HTS 96 Kit/ Tec is designed for rapid and economical automated purification of highly pure genomic DNA from tissue samples or eucaryotic cells in a 96 well format for use on the Freedom Evo from Tecan.

Starting Material

tissue, rodent tails, mammalian cells and paraffin embedded tissue

Product Characteristics

Advantages

• ultra-pure genomic DNA for enhanced performance in sensitive downstream applications
• no cross contaminations
• reproducible yields
• CE-marked in compliance with the Directive 98/79/EC on in vitro diagnostic medical devices (IVD-Directive)*

Overview

Direct lysis** of tissue samples with Proteinase K (at 52 °C) can be performed on the robotic platform, avoiding mechanical homogenization. The lysis efficiency can be dramatically advanced by permanent shaking of the 96 well plate during the lysis step. The lysate is automatically mixed with binding buffer to adjust the optimal binding conditions and the DNA is bound to the membrane of the filter plate under non-chaotropic conditions. Contaminants are removed by washing twice and the ultra-pure DNA is eluted in a low-salt buffer. The purified genomic DNA is of high quality and can be used for a broad panel of subsequent downstream applications:

• PCR
• real-time PCR
• RFLP analysis
• restriction enzyme digestion
• hybridization
• sequencing
• cloning

*) Products which are CE-marked according to the IVD-Directive can be used for diagnostic applications in countries where this directive is recognized.

**For difficult to lyse samples, to reduce processing time or to prevent the risk of any clogging of the tips or needles of the workstation a customized protocol can be prepared including e.g. a homogenization step of the lysate on a mixer mill using Zirconia Beads. Here is it recommended to remove unlysed material and the Zirconia beads by a brief centrifugation step

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